Decoding DNA labels by melting curve analysis using real-time PCR
نویسندگان
چکیده
منابع مشابه
Decoding DNA labels by melting curve analysis using real-time PCR.
Synthetic DNA has been used as an authentication code for a diverse number of applications. However, existing decoding approaches are based on either DNA sequencing or the determination of DNA length variations. Here, we present a simple alternative protocol for labeling different objects using a small number of short DNA sequences that differ in their melting points. Code amplification and dec...
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Real-time monitoring of PCR is one of the most important methods for DNA and RNA detection widely used in research and medical diagnostics. Here we describe a new approach for combined real-time PCR monitoring and melting curve analysis using a 3' end-blocked Exciton-Controlled Hybridization-sensitive fluorescent Oligonucleotide (ECHO) called Eprobe. Eprobes contain two dye moieties attached to...
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A rapid competitive PCR method was developed to quantify DNA on the LightCycler. It rests on the quantitative information contained in the melting curves obtained after amplification in the presence of SYBR Green I. Specific hybridization probes are not required. Heterologous internal standards sharing the same primer binding sites and having different melting temperatures to the natural PCR pr...
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Cryptosporidium spp. are protozoan parasites that infect various vertebrate hosts. Although 10 species are recognized, at least five types have been found to infect human(1). Thus, the identification of Cryptosporidium spp. and genotypes is important for the assessment of public health issues and for tracking infection or contamination sources(2). Presently, the identification of Cryptosporidiu...
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Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical. We developed a real-time fluorescence-based multiplex PCR for the detection of al...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2017
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114618